SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Library quantification: opinions? krobison Sample Prep / Library Generation 41 06-23-2016 06:38 PM
Library quantification suludana Illumina/Solexa 22 10-24-2013 03:52 PM
Illumina library quantification deepakpatilp Illumina/Solexa 75 06-24-2013 09:21 AM
RNA-seq from oligo(dT) primed cDNA ETHANol RNA Sequencing 1 11-23-2011 10:37 AM
Library Quantification Confusion! peromhc Sample Prep / Library Generation 9 10-05-2011 07:18 AM

Reply
 
Thread Tools
Old 06-26-2009, 05:27 AM   #1
Rosanne82
Junior Member
 
Location: Germany

Join Date: May 2009
Posts: 1
Default 3'UTR library or random primed cDNA library for quantification?

I am quite new in the field of next generation sequencing and I hope to get some information and help here.
I want to do a RNA-Seq by using the Illumina platform to analyze the transcript profile of a specific tissue of a wildtype plant compared to a mutant. The aim is identifying a different transcription level of some genes as well as finding some rare genes. The organism I am working with is not sequenced yet, but it should be till the end of this year.
My question is which type of library is more suitable for quantification, a random primed cDNA library or a 3’UTR library, which proposed me a company. But what would be the advantage of a 3’UTR library?

Thank you very much!
Rosanne82 is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:33 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO