Hi,
using a HiSeq device I plan to do amplicon sequencing on a fragment isolated from a plasmid library following restriction digestion with a homing endonuclease. As such, the fragments to be deep-sequenced contain a 11/15 base-long sequence at its 3' end, preceding the adapter sequence. Would that cause any problems at any steps during sequencing, specifically, in attaching them efficiently to the flow cell?
using a HiSeq device I plan to do amplicon sequencing on a fragment isolated from a plasmid library following restriction digestion with a homing endonuclease. As such, the fragments to be deep-sequenced contain a 11/15 base-long sequence at its 3' end, preceding the adapter sequence. Would that cause any problems at any steps during sequencing, specifically, in attaching them efficiently to the flow cell?
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