Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Genewise program output file discussion

    Does anybody familiar with genewise program and know how to save the Genewise program output file into a new file?
    I got two input file now:
    1. A single dna fasta sequence of genome.fasta.masked (output file of RepeatMasker program)
    2. Unitprot.fasta (protein database without formatted, in fasta format)

    My purpose is wanted to run my genome.fasta.masked sequence against the unitprot fasta. Whether I needed to format my unitprot.fasta file by using NCBI "formatdb" before running the genewise program?
    In my case, whether "genewise" or "genewisedb" more suitable to use to map my genome.fasta.masked sequence against the unitprot.fasta database?
    Besides that, how I can save the output result of genewise program?
    Command that I have tried:
    /genewise/wise2.2.0/src/bin/genewisedb -prodb unitprot.fasta -dnas genome.fasta.masked -gff > output_result.gff
    I plan to output the genewise program in gff format then save it to a file called (output_result.gff)
    Thanks.

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
39 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
41 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
35 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X