Hi,
I sequenced several bacterial strains from the same species using Illumina Hiseq 2500 (multiplexing). I then aligned the cleaned reads for each sample to a reference genome with Bowtie2. I observed an important variation in average coverage depth between samples ranging from 70X to 700X for one sample.
Do you have any idea what could be the source(s) of such variation?
Thanks
I sequenced several bacterial strains from the same species using Illumina Hiseq 2500 (multiplexing). I then aligned the cleaned reads for each sample to a reference genome with Bowtie2. I observed an important variation in average coverage depth between samples ranging from 70X to 700X for one sample.
Do you have any idea what could be the source(s) of such variation?
Thanks
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