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  • 100 Gb Data/Day – Nextseq 500 Sequencing Services Now Available on Genohub

    We're pleased to announce you can order NextSeq 500 sequencing services on Genohub.com today: https://genohub.com/shop-by-next-gen...48c30d3f93d505

    We expect the highest interest to be centered around targeted sequencing (exome or custom regions) and fast RNA profiling. For exome studies, you can run between 1-12 samples in a single run and get back 4 Gb at 2 x75 or 5 Gb at a 2×100 read length. If you’re interested in RNA profiling at 10M reads per sample, you can multiplex between 12-36 samples together in a single run. A 1×75 cycle run takes as few as 11 hours to complete and 2×150 runs take ~29 hours.

    For more details: http://blog.genohub.com/100-gb-of-da...le-on-genohub/

  • #2
    Any idea if the NextSeq is more MiSeq or HiSeq-like in its tolerance for low-diversity reads (16S, small number of amplicons, etc)?
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

    Comment


    • #3
      The NextSeq is less tolerant than HiSeq for low-diversity reads. NextSeq uses a two channel SBS sequencing process. It's easier for current software versions to differentiate cross-talk matrix with 4 dyes. MiSeq is still the way to go for amplicon-seq, 16S. We've written a few words about it here:
      Overview of major differences between sequencing platforms/instruments, and comparison of number of reads, read type, read length and error.


      - Genohub

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      • #4
        Good thoughts.
        Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

        Comment

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