SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
BS-Seq from a single cell? eslondon Epigenetics 1 07-26-2011 11:43 PM
Library for single cell RNA seq??? zianeffy Sample Prep / Library Generation 2 07-05-2011 12:46 PM
Normalizing Single Cell libraries apratap Bioinformatics 0 04-07-2011 02:35 PM
Question of small quantity of cell or single cell sequencing hxygz Sample Prep / Library Generation 1 07-16-2009 11:48 PM
mRNA-Seq whole-transcriptome analysis of a single cell. benimmyeo Literature Watch 0 04-26-2009 11:12 PM

Reply
 
Thread Tools
Old 10-29-2010, 08:41 AM   #1
pie
Junior Member
 
Location: UK

Join Date: Jul 2010
Posts: 3
Default RNAseq from single cell RT?

hi,
i am working with a very limited amount of laser captured cells. RNA purification is extremely problematic in this setting, but direct lysis/cDNA amplification works very well.
how should i proceed from this first "classical" RT step to NGS?
thanks
pie
pie is offline   Reply With Quote
Reply

Tags
library preparation, rnaseq, single cell

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:23 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO