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Old 10-03-2018, 01:14 PM   #1
lilpalmitate
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Location: North America

Join Date: Oct 2018
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Unhappy Feasible cryopresevation protocol for simultaneous RNA- and ATAC-seq: solid tissues?

Hi,

I was wondering if anyone has a good protocol to share in terms of cryopreserving solid tissues for both RNA-seq and ATAC-seq later? I am reading that it is better to slow-freeze for ATAC-seq, while adding RNAlater would not be good for ATAC-seq. Is this feasible or would the best way be to divide the tissue up and preserve them differently?

I did try omni-ATAC on flash-frozen tissue and had bad results with very little nuclei yield, I've been advised to try with more tissue, but was wondering if there is an ideal way to prep both libraries from the same tissue without too much material loss or quality compromised. Thanks...!
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