Analysis of RNA-seq data without replicate.

[Sora Yoon]

Hi guys!

Is there any reliable method for statistical analysis of RNA-seq data with no replicates?? If there are only one test and control group, how can I get the p-value of each gene's differential expression??

Thank you for reading.

[dpryan]

There's no truly reliable way to do that because there can't be. The best you can do would be tools like GFOLD.

[mbblack]

Hi guys!

Is there any reliable method for statistical analysis of RNA-seq data with no replicates?? If there are only one test and control group, how can I get the p-value of each gene's differential expression??

Thank you for reading.

A p-value tells you something about an observation relative to a distribution of possible data. That means you require a mean and some knowledge of the variance about that mean. Neither can be computed with an N of 1.

[Sora Yoon]

Oh

There's no truly reliable way to do that because there can't be. The best you can do would be tools like GFOLD.

Thank you! I will try GFOLD.