Hi all,
I got a big file of data back from the sequencing centre that worked fine when I put it through fastqc, but after demultiplexing it into the individuals, FastQC complains that the id lines don't start with @. I've used two demultiplexers (Stack's process_radtags and GBSX) and it occurs with both of them.
So the demultiplexing process is causing this, which is odd as it worked fine with the other two datasets I've got, and put through exactly the same process.
Has anyone got experience of why this might be?
Cheers,
Steve
I got a big file of data back from the sequencing centre that worked fine when I put it through fastqc, but after demultiplexing it into the individuals, FastQC complains that the id lines don't start with @. I've used two demultiplexers (Stack's process_radtags and GBSX) and it occurs with both of them.
So the demultiplexing process is causing this, which is odd as it worked fine with the other two datasets I've got, and put through exactly the same process.
Has anyone got experience of why this might be?
Cheers,
Steve
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