Hi all.
I am completly new at NGS. I have just got back my datas from a first run of 454 pyrosequencing. We want to get into metagenomics and we have decided to use mothur.
Before to start with mothur, I'd like to have a look to my results using FastQC. The problem is that I don't have the fastq format, just the sff, fna and qual. I would know how to do that on linux, but we are using windows terminal. Does anybody now how to extract the file on windows? sff_extract doesn't work.
By the way I would like to know which one would be the next step we must follow, I mean, from my demultiplexed samples. I guess it would be the denoising step, but not sure....
Thanks for the help,
Elisa
I am completly new at NGS. I have just got back my datas from a first run of 454 pyrosequencing. We want to get into metagenomics and we have decided to use mothur.
Before to start with mothur, I'd like to have a look to my results using FastQC. The problem is that I don't have the fastq format, just the sff, fna and qual. I would know how to do that on linux, but we are using windows terminal. Does anybody now how to extract the file on windows? sff_extract doesn't work.
By the way I would like to know which one would be the next step we must follow, I mean, from my demultiplexed samples. I guess it would be the denoising step, but not sure....
Thanks for the help,
Elisa
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