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Old 03-21-2020, 06:13 PM   #1
Ichisen
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Location: Philippines

Join Date: Mar 2020
Posts: 1
Post [NEWBIE] Interpretation of blastp results

I'm extremely new to this and I apologize if this question seems naive. I created a database for virulence factors and did BLASTP.

I got the the following values:

qaccver saccver pident length mismatch gapopen qstart qend sstart send evalue bitscore

However, according to my reference journal, I need "slen" or length of matching sequence and "qlen" or length of query sequence to be used in a formula to identify the homologous protein. The formula is:
Ha = pident x slen/qlen

Is "slen/qlen" equivalent to "length" already?

or can I just use evalue and pident to identify the best match? Can you recommend a protocol for this?

Thank you!
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blast, blastp, evalue cutoff, homology, length

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