Hello All,
I am confused about what an Insert actually is ? And what is an insert size ?
I am dealing with 454 paired end data. How is a Linker different from an Adaptor?
I will be using MIRA's sff extract to extract the fasta and qual files from the sff files.
So does the sff files have read info this way:
|-----75----|------------------------100-----------------|-----75-----|
i.e - Seq.forward - Linker - Seq.reverse ??
In the above, what is an insert ? and whats the size ?
So do each of the reads in an sff have the above format ?
So in the MIRA's mates.file setup can I just use the following default format for the mate pairs ?
pair (.*)\.f (.*)\.r
Please help me out with my confusions:
Thanks
Aarthi
I am confused about what an Insert actually is ? And what is an insert size ?
I am dealing with 454 paired end data. How is a Linker different from an Adaptor?
I will be using MIRA's sff extract to extract the fasta and qual files from the sff files.
So does the sff files have read info this way:
|-----75----|------------------------100-----------------|-----75-----|
i.e - Seq.forward - Linker - Seq.reverse ??
In the above, what is an insert ? and whats the size ?
So do each of the reads in an sff have the above format ?
So in the MIRA's mates.file setup can I just use the following default format for the mate pairs ?
pair (.*)\.f (.*)\.r
Please help me out with my confusions:
Thanks
Aarthi
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