Hi guys,
I'm a newbie about the ATAC-seq, maybe here there is someone more expert than me.
I'm doing an ATAC-seq on my samples (I have 3 different samples form thymic epithelium), and I'm a bit stucked about how to amplify my tagmented DNA. I've seen the primers list from Greenleaf lab (Supplemental table from the article), but now I have no idea of how to use those primers. Do I have to use all of them? I need to mix them?
Sorry if it's a stupid question. Science is infinite knowledge!
thank you very much for your help.
I'm a newbie about the ATAC-seq, maybe here there is someone more expert than me.
I'm doing an ATAC-seq on my samples (I have 3 different samples form thymic epithelium), and I'm a bit stucked about how to amplify my tagmented DNA. I've seen the primers list from Greenleaf lab (Supplemental table from the article), but now I have no idea of how to use those primers. Do I have to use all of them? I need to mix them?
Sorry if it's a stupid question. Science is infinite knowledge!
thank you very much for your help.
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