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Old 09-01-2017, 10:46 AM   #1
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Location: USA

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Default Doubt for primer removal: illumina

Hi All,

I was browsing online bioinformatics forum and stumbled onto query about primer, adapters that put me in thought about the process/pipeline I'm performing. I've Illumina paired-end 100bp, whole-genome bacterial data.

I assemble these reads using denovo assembler, things look good. But in an earlier step I trim these using trimmomatic tool, using an adapter file provided by sequencing center.

However, I never remove primers.
I'm wondering why I didn't remove primers, nor did I see any issue in my downstream analysis of ST identification, phylogenetic trees.

Should I not remove primers? Why?
Bioinformaticscally calm
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Old 09-01-2017, 12:47 PM   #2
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See if this primer helps.
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Old 09-01-2017, 03:44 PM   #3
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The primer sequences will match part of the full adapter sequences (or their reverse complement). Thus adapter trimming will usually be just fine. "Free" primers should not show up in your sequence data.
luc is offline   Reply With Quote

adapter, illumina, primer

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