Hi, i am about to order 400 barcoded primers (100 barcodes + 2 directions x 2 Genes of Interest).
Usually i check my primers with the OligoAnalyzer by IDT, but they dont accept the upload of fasta files or so.
My question is if there is a tool which can assess all 400 primers and can give out: Tm, worst Hairpin/selfdimer/crossdimer deltaGs, at my desired PCR parameters?
That would be handy. Thank you!
/edit: I just came across barcrawl. Is it working the way as described above?
Usually i check my primers with the OligoAnalyzer by IDT, but they dont accept the upload of fasta files or so.
My question is if there is a tool which can assess all 400 primers and can give out: Tm, worst Hairpin/selfdimer/crossdimer deltaGs, at my desired PCR parameters?
That would be handy. Thank you!
/edit: I just came across barcrawl. Is it working the way as described above?