Hi all,
I am working on double digest RAD sequencing, and I have quite a general question. In the ddRAD protocol of Peterson et al. they do a beads cleanup directly after enzyme digestion. However, I also know people who skip this step and only do the beads cleanup after the ligation. I am curious what you guys do in your lab and why? I suppose it is a balance between increasing the concentration of the DNA versus loss of DNA?
I would like to make a well-informed decision for my samples
Diede
I am working on double digest RAD sequencing, and I have quite a general question. In the ddRAD protocol of Peterson et al. they do a beads cleanup directly after enzyme digestion. However, I also know people who skip this step and only do the beads cleanup after the ligation. I am curious what you guys do in your lab and why? I suppose it is a balance between increasing the concentration of the DNA versus loss of DNA?
I would like to make a well-informed decision for my samples
Diede
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