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Old 01-31-2013, 07:41 AM   #1
lvenga
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Location: london

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Default transposon/insertion sequencing techniques

Hi,

could anyone briefly summarise the main differences between:

-IN-Seq (Insertion sequencing)
-Tn-Seq (Transposon sequencing)
-HITS (High-throughput insertion tracking sequencing)
-TraDIS (Transposon-directed insertion-site sequencing)
?

Thanks a lot!
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Old 05-30-2013, 01:17 PM   #2
dcnadler
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Location: Bay Area

Join Date: May 2013
Posts: 3
Default

Quote:
Originally Posted by lvenga View Post
Hi,

could anyone briefly summarise the main differences between:

-IN-Seq (Insertion sequencing)
-Tn-Seq (Transposon sequencing)
-HITS (High-throughput insertion tracking sequencing)
-TraDIS (Transposon-directed insertion-site sequencing)
?

Thanks a lot!
There is a review that just came out which should provide the answer: http://www.nature.com/nrmicro/journa...micro3033.html

It has a section that briefly describes the differences and a nice figure to illustrate them.
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