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Old 09-23-2021, 09:08 PM   #1
M Adnan
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Location: Japan

Join Date: Sep 2021
Posts: 1
Default Makeblastdb error: file does not exist.

I just downloaded the blast command line applications and I would like to make a database of a MGEs nucleutides that i downloaded from ACLAME database site in fasta file. It is labeled as mydb.fasta and I have put the file into a bin folder of blast in the path C:\Program Files\NCBI\blast-2.12.0+\bin

Ive been typing this into the command line:

cd C:\Program Files\NCBI\blast-2.12.0+\bin to enter in directory

makeblastdb -in mydb.fasta -dbtype nucl -out DB

Building a new DB, current time: 09/23/2021 13:48:15 New DB name: C:\Program Files\NCBI\blast-2.12.0+\bin\DB New DB title: mydb.fasta Sequence type: Nucleotide Keep MBits: T Maximum file size: 1000000000B BLAST options error: File mydb.fasta does not exist

every time i got this error file does not exist. It is probably a simple problem to solve, but I cannot seem to get past it.
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