I'm new to bioinformatic analysis and statistics, and this is my first post.
I've got a basic question regarding the insert size distribution of paired-end reads.
Most of PEM-based SV callers assuming a normal distribution from insert size.
I have made a plot with my own data (76bp PR from MiSeq) and I see that this distribution is right skewed. So my question is, are these callers appropiate for skewed distributions? Are they performing any data transformation? I'm a bit confused,
Thanks
I've got a basic question regarding the insert size distribution of paired-end reads.
Most of PEM-based SV callers assuming a normal distribution from insert size.
I have made a plot with my own data (76bp PR from MiSeq) and I see that this distribution is right skewed. So my question is, are these callers appropiate for skewed distributions? Are they performing any data transformation? I'm a bit confused,
Thanks
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