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Old 09-04-2013, 05:01 PM   #1
lucialucia11678
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Default QC with BioA following second strand?

I am in process of preparing libraries with 10 ng and 100 ng mRNA for RNA-Seq. I ran a BioA chip (DNA HS) following second strand synthesis and bead purification. However my BioA traces are flat? At this level of mRNA input would I expect to see a library peak following ds cDNA? I just wanted a quick QC before proceeding along the LC process. Any ideas are welcome!
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Old 09-09-2013, 11:48 AM   #2
Isequencestuff
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Maybe looking at this thread might give you some insight?

http://seqanswers.com/forums/showthread.php?t=2003
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