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Old 07-15-2015, 12:50 PM   #1
SDK
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Location: Canada

Join Date: Jan 2014
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Default Squeezing the most out of Nextera XT...?

Hi All,

I'm wondering if anyone has experimented with pushing the limits of Nextera XT in order to get more samples out of a kit? I want to sequence a bunch (~150) of mitogenomes generated via long-range PCR. I was hoping to find a way to stretch the Nextera XT kit (and indices) to get the most bang for my buck. Since the samples are amplicons I think I should have enough "room" on one MiSeq run to collect all 150 mitogenomes at high depth...but don't want to have to pay for 150 Nextera XT libraries. Any ideas? Can I just run smaller reactions of the Nextera XT sample prep kit?

Thanks for any ideas.
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Old 07-16-2015, 08:11 AM   #2
alec
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Have a look at this paper: http://journals.plos.org/plosone/art...l.pone.0128036
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amplicon, illumina, miseq, mitogenome, nextera

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