Hi All,
New member here. I had a dig around with google but was unable to come up with an answer. I have a quick question with regards to the traditional Sanger Sequencing versus Next Generation Sequencing. I want to get a feel for the percentage of rRNA reads going into NGS after knockingdown rRNA in my sample. Would doing it the traditional way (i.e. cloning, ligating, transforming, picking colonies for sequencing) by picking say 20 colonies off a plate and sequencing be a good estimate of the percentage of rRNA background in my sample, or would this introduce bias and I would be better off doing a trial run for NGS?
Your thoughs, suggestions would be gratly appreciated.
Cheers,
Darren
New member here. I had a dig around with google but was unable to come up with an answer. I have a quick question with regards to the traditional Sanger Sequencing versus Next Generation Sequencing. I want to get a feel for the percentage of rRNA reads going into NGS after knockingdown rRNA in my sample. Would doing it the traditional way (i.e. cloning, ligating, transforming, picking colonies for sequencing) by picking say 20 colonies off a plate and sequencing be a good estimate of the percentage of rRNA background in my sample, or would this introduce bias and I would be better off doing a trial run for NGS?
Your thoughs, suggestions would be gratly appreciated.
Cheers,
Darren
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