This is my first time in using the TruSeq library Nano LT preparation kit, and also I was using ezRAD method, digesting the DNA samples using Ecor I and then followed the protocol in the TruSeq Nano Kit. However, the final library fragments are much larger, 600-1100 bp, than expected 500-600 bp. Any suggestions on how to improve the method? Anyone have any experiences in getting larger fragment sizes when using the new TruSeq Nano kit? Thank you so much.
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TruSeq Nano Library kit produced largger fragemnt sizes: 600-1100 bp
Last edited by Dongmei_MPI; 10-02-2016, 01:57 AM.
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