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Old 09-08-2015, 06:58 AM   #1
ticirocha
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Location: Italy

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Default Evaluating RAST annotation

Hi everyone,
I'm doing an annotation using RAST in a non-ordered contigs genome and I'm having trouble to determine if the annotation was done properly or not, because the reference genomes avaible are not that close to my microorganism... so I have almost half of my CDS not agrouped in subsystems, and also, I can't tell how bad it is.
How can I evaluate the quality of the annotation? How can I decide if a CD is properly annotated or not?
Can anyone help me, please ?
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Old 09-10-2015, 12:24 PM   #2
cliffbeall
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I did something like this recently and about 40% of genes did not get a COG assignment. One way to evaluate the quality would be to check for a list of core housekeeping genes to make sure they are present and correctly annotated.
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Old 09-10-2015, 01:21 PM   #3
ticirocha
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Thank you Cliffbeall! That's what I did... the ones that interests me are there and well annotated... I think I'll move on like that...
thanks again!
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