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We have a eukaryotic diploid genome assembled and scaffolded using ALLPATHS-LG. In addition, we have a linkage map generated from iso-crossing individuals and performing reduced-representation (GBS) sequencing. We have evidence from the linkage map that can place and order our scaffolds, or at times nest a scaffold within a scaffold gap of a larger scaffold, but it would be a lot of work to manual review these joins and investigate mate-pair evidence across the entire genome. I was wondering if there was any existing pipelines or software programs that generate superscaffolded assemblies based off of a mix of linkage map information and mate-pair information. I have seen several papers, but their methods are either not clearly described, or are very manual in nature, but it is likely I could be missing something.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM