Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • cummerBund -> Doesn't see my samples

    I have 3 samples in 3 folders under the working directory of R, I did "Change directory" and pointed out one new folder "data" under the root C. "Data" contains our 3 samples outputed from cuffdiff.
    • ~/Sample1
    • ~/Sample2
    • ~/Sample3


    How can I point to R the directory for each sample in one readCufflinks command?
    If I just call readCufflinks() it returns 0 sample ..

    Code:
         > cuff<-readCufflinks()
         > cuff
         CuffSet instance with:
             0 samples
             0 genes
             0 isoforms
             0 TSS
             0 CDS
             0 promoters
             0 splicing
             0 relCDS


    I need to execute the following, and retrieve cuff, dispersion graph, density graph and boxplot graph:

    Code:
    > cuff<-readCufflinks(system.file("extdata", package="cummeRbund"))
    > cuff 
    
    > disp<-dispersionPlot(genes(cuff))
    > disp
    
    > genes.scv<-fpkmSCVPlot(genes(cuff)) 
    > isoforms.scv<-fpkmSCVPlot(isoforms(cuff))
    
    > dens<-csDensity(genes(cuff)) 
    > dens
    
    > b<-csBoxplot(genes(cuff)) 
    > b

  • #2
    Code:
    setwd('Sample1')
    cuff1 <- readCufflinks()
    setwd('../Sample2')
    cuff2 <- readCufflinks()
    setwd('../Sample3')
    cuff3 <- readCufflinks()

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    59 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    57 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    53 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    56 views
    0 likes
    Last Post seqadmin  
    Working...
    X