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Old 12-07-2010, 08:54 AM   #1
snowkat
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Location: Maine

Join Date: Dec 2010
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Smile Grad Student in way over my head!

Hi,
I'm new here. Just trying to get my bearings to see what I can find out about using paired end reads to detect structural variation in tumor genomes. I know it can be done... just not sure what path to take and whether or not I can do it myself or if I will need to find someone to collaborate with.

Cheers!
Kathy
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Old 12-07-2010, 11:23 AM   #2
husamia
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are you referring to human genomes? you need to narrow down little bit your target of interest. its difficult to screen entire genome for structural variants and how many samples are you considering?
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Old 12-07-2010, 11:27 AM   #3
snowkat
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Hi,
We are interrogating the mouse genome using paired end mapping. We want to find new translocation junctions... so narrowing regions or enriching would prohibit that. We are looking at running a handful of samples, up to 10.
Are you thinking I am going to have problems with this approach?
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Old 12-07-2010, 11:59 AM   #4
NextGenSeq
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I think your approach is fine if you can afford it. The analysis might be difficult though.
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Old 12-07-2010, 03:21 PM   #5
frozenlyse
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I saw a talk from a guy from the Sanger who does this routinely in human clinical samples - they say they (pretty much) find all structural variants by fragmenting DNA to 600bp and then running 2 lanes of 50bp PE sequencing on a HiSeq
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Old 12-07-2010, 04:10 PM   #6
snowkat
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Quote:
Originally Posted by NextGenSeq View Post
I think your approach is fine if you can afford it. The analysis might be difficult though.
I have read that there are programs (such as PEMer) that can help with the analysis... any experience with it? Heard any horror stories?
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