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I have a sequenced paired end data of S.cerevisisae, sequenced by Illumina, I was wondering why there is low coverage in the centromeric regions compared to the rest of the genome. There are centromeric regions that even do not have any coverage. My average coverage is 30, and the reads are 100bp long. The fragments that was sequenced had a length of 600bp (reads are 100bp form one end and 100bp from other , paired reads)
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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