We have many vials that contain mixture of yeast STRAINS (of 3 or more strains). Currently we are using Capillaries Electrophoresis (CE). With culturing based method for isolation.
And to identify these strains we use microsatellite(STR) by amplifying 7-loci in multiplex PCR. So these are 7loci are non-overlapping and can be distinguished by different dye color in CE.
At the end of the day; we can find out how many&what strains are in each vial and in what proportion by comparing these microsatellite fingerprint to our database.
I am just wondering if there is a way we can use illumina or NGS to do this with much higher resolution and non-culturing method. Thus our current method current resolution are based on how many colonies we decided to isolate.
Our project are quite similar to forensic, but that we have mixture of different strains(individual if human).
Thanks!!
And to identify these strains we use microsatellite(STR) by amplifying 7-loci in multiplex PCR. So these are 7loci are non-overlapping and can be distinguished by different dye color in CE.
At the end of the day; we can find out how many&what strains are in each vial and in what proportion by comparing these microsatellite fingerprint to our database.
I am just wondering if there is a way we can use illumina or NGS to do this with much higher resolution and non-culturing method. Thus our current method current resolution are based on how many colonies we decided to isolate.
Our project are quite similar to forensic, but that we have mixture of different strains(individual if human).
Thanks!!
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