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Old 06-11-2011, 07:36 AM   #1
HerefordGuy
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Location: Missouri

Join Date: Apr 2010
Posts: 6
Default SGA preprocess error, read ids

SGA preprocess is giving me the following error:

Warning: Pair IDs do not match (expected format /1,/2 or /A,/B)
Read1 ID: HWUSI-EAS558R_0015:7:26:4067:7956#0/1
Read2 ID: HWUSI-EAS558R_0015:7:26:4067:7956#0/2

When I forgot and left the --phred64 tag off, it thought the ends of the pair ids were part of the quality scores.
My question, are the read ids too long for SGA? If so how would I change this? Or is this a problem with Bamtools, which SGA uses?

Thanks!
Jared
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