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Old 06-24-2011, 10:21 AM   #1
jbertsche
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Location: Virginia

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Default emPCR on thermal cycler

Hi Everyone,

I know that the Roche manual says that the emPCRs can sit at 10 degC on the thermal cycler for up to 16 hours. I was wondering if any one has any experience with leaving them on for longer?
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Old 07-07-2011, 06:22 AM   #2
crndy52
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I have started emPCRs and put them on the thermal cycler on Friday afternoon and left them until Monday morning when I started the breaking. I suppose this could make a difference, but I could not see any difference in my bead recovery rate or the run data when compared to other runs of the same samples.
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Old 07-07-2011, 07:52 AM   #3
TonyBrooks
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I haven't left them for as long as a weekend, but I've left them 20 hours or so with no problems.
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