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Old 05-22-2012, 07:02 AM   #1
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Default SureSelect bait QC via RNA-seq ?

Anyone done it or thought about it?

I'm very interested in finding out if the gaps in my coverage are caused by the baits just not being efficiently synthesized/transcribed. This is one explanation for the LOW variability in baits from the same array printing, but HIGH variability between different printings of the same content.

I know I could probably do individual RT-PCRs, but I'd prefer to just massively query the whole bait population.

I suppose the bio-UTP could affect RT activity, and it's very possible Im missing something else...but just wanted to throw this out there for discussion.
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Old 05-22-2012, 11:04 AM   #2
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I am curious to know as to how many gaps are you finding in the coverage? What is the size of locations you are interrogating?
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