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Old 04-01-2013, 02:58 AM   #1
bernardo_bello
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Default dUTP/UDG or RNA ligation sequencing method?

Hi community,

I would like to know the strand specificity type of RNA libraries prepared with Ion Total RNA-Seq Kit v2 - Life Technologies protocol.

Thank you.

Regards, Bernardo
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Old 04-01-2013, 03:26 AM   #2
GenoMax
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See if this has what you need: http://tools.invitrogen.com/content/...s/4476286B.pdf
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Old 04-01-2013, 04:48 AM   #3
bernardo_bello
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Quote:
Originally Posted by GenoMax View Post
Thank you GenoMax,

Which is the difference between dUTP/UDG and RNA ligation sequencing method?

Bernardo
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Old 04-01-2013, 04:25 PM   #4
snetmcom
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One converts to cDNA and digests away a strand to maintain directional infomration.

The Ion kit ligates dsAdapters directly to RNA, which typically has better results.
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Old 04-02-2013, 03:55 AM   #5
bernardo_bello
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Quote:
Originally Posted by snetmcom View Post
One converts to cDNA and digests away a strand to maintain directional infomration.

The Ion kit ligates dsAdapters directly to RNA, which typically has better results.

Thank you snetmcom,

Now it's clear.

I found in this paper the dUTP/UDG method explained, althoght the Ion Torrent RNA-seq library preparation is based on ligation method.

http://www.ncbi.nlm.nih.gov/pubmed/21943893
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