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has anyone used a hotspot file yet? Rabu Ion Torrent 0 06-02-2015 09:02 AM

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Old 04-01-2016, 06:05 AM   #1
tristan dubos
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Location: France

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Default AcroMetrix Oncology Hotspot

Hi all ,
I'm working in result from the sequencing of AcroMetrix Oncology Hotspot on PGM iontorrent to validate our pipeline. In the list of the mutation i have a strange case : screen_shot_mut_chr4_1807869.png

As you can see there 50 reads with the mutation in foward position but only 2 reads on the reverse position. I use a strand bias in my pipeline that is why i did not find the mutation in my results. But finally i do not understand why there is a difference between foward and reverse reads.

Do you have any ideas ? It-is an library bias ?

Kind regard

Tristan
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