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Old 04-11-2013, 07:10 AM   #1
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Location: USA

Join Date: Feb 2012
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Default Using TruSeq adaptors for custom protocol?

We are currently using Illumina's TruSeq RNA Sample Prep Kit and are considering doing a homemade/custom protocol instead. We have tons of the TruSeq adaptors left over from the kits and we were wondering if we could use these adaptors with a homemade protocol. We would most likely use Phusion in the cDNA enrichment step if this makes a difference.

Any help or suggestions would be appreciated!
mpaulsen is offline   Reply With Quote
Old 04-11-2013, 08:29 AM   #2
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As long as you are adding them to double stranded DNA or cDNA that has been prepared with end repair and A tailing it should work.
kwaraska is offline   Reply With Quote

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