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Old 10-31-2013, 08:38 AM   #1
MonaE
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Location: France

Join Date: May 2013
Posts: 24
Default Size selection...dropping of DNA quantity

Dear all,

My samples quantities was 246, 184, 112, 133 and 232 ng. I pooled them all and run them in a gel for site selection. Afterward I did purified them using columns. Surprisingly and after ,measuring them with Qubit their quantity became 10.7 ng.

First, I would like to know why they have such a low concentration?

second, if the beads are the reason, then how can I avoid this?

Third, will these samples still work to proceed with? for Ion one touch?
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