Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
MiSeq: V1-2 vs V4 for 16S rRNA amplicon sequencing fish Illumina/Solexa 23 07-21-2016 04:02 AM
amplicon sequencing on MiSeq nancysch Illumina/Solexa 20 03-11-2015 10:29 AM
Miseq Amplicon sequencing library prep help clmcmurray Illumina/Solexa 2 03-18-2014 12:09 AM
Amplicon based sequencing on the MiSeq nvpatkar Illumina/Solexa 4 10-29-2012 05:41 AM
Amplicon based sequencing for the MiSeq nvpatkar Sample Prep / Library Generation 0 09-27-2012 02:24 PM

Thread Tools
Old 03-17-2014, 08:46 PM   #1
Location: seattle

Join Date: Aug 2012
Posts: 38
Default storage of Amplicon Sequencing products for MiSeq

Greetings, i have a very basic question. I 18 samples that have been PCR amplified with barcoded primrs, cleaned, and have been stored at 4 degrees C for a few days. Will the quality of the barcodes decrease at 4 degrees versus -20? If anyone has any experience with running these types of experiments, and wouldn't mind sharing, i would appreciate it.

tonybert is offline   Reply With Quote
Old 03-17-2014, 10:49 PM   #2
Location: CA

Join Date: Jul 2013
Posts: 74

We've been wondering about this too recently. We also have a lot of sunlight in our lab and our 4C fridge has a glass door so we've started to keep libraries in boxes or wrapped in foil. Would be interested to see what others have experienced.
atcghelix is offline   Reply With Quote
Old 03-19-2014, 02:52 AM   #3
Location: London

Join Date: Nov 2012
Posts: 96

DNA should be fine for months at 4 degrees, provided there's no nuclease contamination.
JamieHeather is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:21 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO