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Old 03-11-2010, 02:12 AM   #1
Junior Member
Location: Ludhiana, India

Join Date: Jan 2010
Posts: 7
Default Need Help Regarding Reciprocal best hit in BLAST

Hello everyone


I need to know about the syntax used for BLAST using Reciprocal Best Hit method. What parameters should I use for it.
If you have some time.... then please provide me links for reading about Reciprocal Best Hit method.

Thanks in anticipation
Regards from
Bharat is offline   Reply With Quote
Old 03-11-2010, 03:04 AM   #2
Peter (Biopython etc)
Location: Dundee, Scotland, UK

Join Date: Jul 2009
Posts: 1,543

I don't understand your question. Reciprocal Best Hit isn't something built into the BLAST tools. Most people doing a BLAST Reciprocal Best Hit (RBH) do this with a script in Perl/Python/... You just run BLAST with suitable parameters twice, first using list A as the queries and list B as the database, and then the other way round.

You should be able to find examples of BLAST RBH in the literature.
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