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Old 01-08-2015, 08:39 PM   #1
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Location: uk

Join Date: Dec 2011
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Default (seeking help)zero cluster PF density.

The 1-4 Lane we used inhouse target sequencing first time(similar like SureSelectQXT Target Enrichment), 5-8 Lane were normal exome sequencing, and we used Treseq SBS V3.
The cluster density looks fine but the PF density was terrible. we don't what caused it.
We didn't add PhiX in the 1-4 Lane.
Is there anyone knows what the problem is?
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target cluster pf

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