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Old 12-17-2015, 01:35 PM   #1
Junior Member
Location: San Diego, CA

Join Date: Oct 2015
Posts: 1
Default How often are you washing your MiSeq?

Curious to see how often other users are washing their MiSeqs. We are currently doing a maintenance wash with 0.5% tween every Monday morning, but sometimes the instrument doesn't get used until Thursday/Friday so I'm considering a post run wash in between the days.
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Old 12-18-2015, 08:59 AM   #2
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Location: Washington, D.C. metro area

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On a schedule like that, I'd do your maintenance wash Monday and then a post-run wash the day you start your sequencing run. If you start the wash when you pull out the cartridge to thaw, you should have plenty of time.
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Old 12-18-2015, 09:07 AM   #3
Location: Germany

Join Date: Jun 2011
Posts: 10

Recommended washes are outlined in the MiSeq User Guide.
Please find the relevant section attached.
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