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Old 08-21-2018, 05:30 AM   #1
Junior Member
Location: Cape Town, South Africa

Join Date: Aug 2018
Posts: 1
Question Wierd base calling on MiniSeq run

We recently ran some libraries on the MiniSeq (2 x 150 bp Mid Output kit) and got some weird traces for the %base graph (see image attached). The other metrics on SAV look pretty good.

We prepped libraries from 3 bacterial genomes (C. diff, E. coli and B. per - i.e. low to high GC content) in triplicate using the SureSelect QXT library prep kit.

We didn't perform the capture step of the protocol and amped on the indexes post-ligation.
The kit uses transposase-based fragmentation and requires unique sequencing primers, which we added to the MiniSeq reagent kit. We used both the Illumina primers and the QXT because we included a 1% phiX spike-in.

Has anyone seen similar results?
Attached Images
File Type: png MiniSeq %base_SureSelect QXT.PNG (139.5 KB, 21 views)
AAG_GGA is offline   Reply With Quote

base calling, illumina, run quality, sureselect, transposase

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