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Old 12-01-2008, 07:20 PM   #1
elly
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Default Homemade illumina oligos and library prep kit

Hi,

I am wondering if we can use the PCR amplification primers which were made at our side.
In theory, this can work properly.

But I am worrying a little bit if there can be some problem with sequencing primer binding and PCR amplification step.

Is there anyone who attempt this?

Thank you in advance.
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Old 01-24-2009, 10:39 AM   #2
graveley
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Yes, as long as the sequences you use are the same as the Illumina sequences, primers you synthesize yourself work fine!
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Old 02-02-2009, 08:04 AM   #3
andibody
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Is this also true for adaptors and sequencing primers?

Thanks
Andi
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Old 03-24-2009, 08:05 AM   #4
kkannan
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For the PE PCR primers, is it necessary to PAGE purify them?
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