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I am a newbie to NGS.We are evaluating the possibility of doing CNV analysis by NGS data eigher by Illumina MiSeq or Ion PGM platform on archived melanoma FFPE sample. We donot want high cycle PCR involved in the protocol which may saturate the result. But both Illumina (targetd enrichment) and Ion PGM protocol (template preparation) include high cycle PCR. Does anyone have a good idea? Thanks.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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