Hi all!
I have bacterial metatranscriptome from 12 samples but I don't have any biological replicates. They are a time serie (1 sample for each time point).
I know that for differential expression analysis I would need replicates, but at the moment we can't afford that.
I'd like at least to be able to say if there is a higher or lower expression of some genes during the time.
Anyone can suggest me how to proceed?
How can I normalize the gene counts for sequencing depth and calculate which genes are up or down-regulated between each couple of samples?
Thanks a mil
francesca
I have bacterial metatranscriptome from 12 samples but I don't have any biological replicates. They are a time serie (1 sample for each time point).
I know that for differential expression analysis I would need replicates, but at the moment we can't afford that.
I'd like at least to be able to say if there is a higher or lower expression of some genes during the time.
Anyone can suggest me how to proceed?
How can I normalize the gene counts for sequencing depth and calculate which genes are up or down-regulated between each couple of samples?
Thanks a mil
francesca