Hi,
we have been working for a while with custom barcoded adapters. Successful basecalling seems to depend mostly on sufficient base complexity and we therefore run a minimum of 4 samples per lane.
However, we are testing at the moment illuminas Truseq barcoded adapters and were wondering if they are also prone to camplexity restrictions. Has anybody tested running only 2 or 3 barcodes in a lane? In theory, all basecalling parameters were calculated in read 1 so the reduced complexity shouldn't be such an issue. Any thoughts?
best
A
we have been working for a while with custom barcoded adapters. Successful basecalling seems to depend mostly on sufficient base complexity and we therefore run a minimum of 4 samples per lane.
However, we are testing at the moment illuminas Truseq barcoded adapters and were wondering if they are also prone to camplexity restrictions. Has anybody tested running only 2 or 3 barcodes in a lane? In theory, all basecalling parameters were calculated in read 1 so the reduced complexity shouldn't be such an issue. Any thoughts?
best
A
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