Quality of 150 bp

[Brugger]

Hi

I am looking for some 150bp illumina data to examine the quality of the read at the end. If this is not possible I would be very happy for a QV box-plot showing how the sequence quality drops off at the end of the read, or just hear of anybodies experience 150bp long reads.

Cheers,

[tonybolger]

I am looking for some 150bp illumina data to examine the quality of the read at the end. If this is not possible I would be very happy for a QV box-plot showing how the sequence quality drops off at the end of the read, or just hear of anybodies experience 150bp long reads.

We have run two lanes of 2x150bp - I can't share the data, or probably even the plots, but i can give you the highlights.

The best lane was still median ~Q30 at the end of the forward reads, the other lane was a few bases worse, ~Q30 at position 146, dropping to the mid 20s by the end. The reverse reads from each lane were somewhat worse than the corresponding forward reads, degrading 5-10 bases earlier.

Compared against our better 2x100bp runs, the 150 long reads are somewhat better in the corresponding positions - Q36 at position 100.

[dmborek]

which instrument?

Was it GAII or HiSeq? What was clusters density?

D.