Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • blast2go command line!

    Hello friends,

    I am a new user of blast2go pipeline.

    I would like to use blast2go pipeline to obtain annotation for a blast output file which is about 17 Megabytes in size. I did blast against nr seperately and I have the output as *.xml file from my blast. We have a 16 core linux machine and I would like to run blast2go pipeline in that linux machine.

    I am not sure if there is an option for setting the number of threads while running blast2go pipeline.

    The b2g4pipe command that I used on linux command line to test a small sample blast output is

    nohup java -Xmx1000m -cp *:ext/*: es.blast2go.prog.B2GAnnotPipe -in 10_BlastResults_2011.xml -out b2gpout -prop b2gPipe.properties -annot &

    It works fine and I could get the output without any problem.

    For running b2g4pipe on a larger blast output file in xml format:

    Could you please let me know if I could set the number of threads(no of cores/cpu's) in the above b2g4pipe command( like how we do in blastx command line with -num_threads option) to make the annotation process faster? If not, then is there any other way to do it?


    Thanks very much.

    Cheers,
    PSW.

  • #2
    The best way to speed up Blast2Go or b2g4pipe is to setup a local Blast2GO database rather than connecting to their public database in Spain.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 08:47 AM
    0 responses
    16 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    54 views
    0 likes
    Last Post seqadmin  
    Working...
    X