Hi
I am trying to run an Illumina MiSeq reaction in a tube. Does anybody know which dyes correspond to each particular (A, T, G , C) reversible terminator.
Did anybody try to run the reaction in a tube/PCR machine. Any advice on a reference where the conditions (cycle duration, temperature) are mentioned would be highly appreciated.
Thanks in advance!!
I am trying to run an Illumina MiSeq reaction in a tube. Does anybody know which dyes correspond to each particular (A, T, G , C) reversible terminator.
Did anybody try to run the reaction in a tube/PCR machine. Any advice on a reference where the conditions (cycle duration, temperature) are mentioned would be highly appreciated.
Thanks in advance!!