Hello.
Our lab previously used 454 pyrosequencing to analyse 16S rDNA data. We have recently moved to using MiSeq (we get more samples done in one run). However we have come across a problem. There is a large discrepancy between one MiSeq run and the next.
We have tried to rarefy the data to minimize this difference, but it has made little or no difference.
Has anyone seen such a run bias? Can anyone suggest any programs or anything that might be able to reduce it?
Thank you in advance!
Our lab previously used 454 pyrosequencing to analyse 16S rDNA data. We have recently moved to using MiSeq (we get more samples done in one run). However we have come across a problem. There is a large discrepancy between one MiSeq run and the next.
We have tried to rarefy the data to minimize this difference, but it has made little or no difference.
Has anyone seen such a run bias? Can anyone suggest any programs or anything that might be able to reduce it?
Thank you in advance!
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