Hi all,
I am looking for advice from anyone who has experience of using the TruSeq Custom Amplicon Library Preparation kit please! Part of the protocol requires a 96well heat block that will cool samples from 95C to 40C over 80 minutes. We don't have the reccomended incubator, and Illumina Tech Support have said we can't use a thermal cycler. Our hot blocks take 240 minutes to cool to 40C - way to long for the samples to work. Has anybody used this kit with anything other that the recomended Scigene Hybex microsample incubator?? - don't want to have to spend out to test the kit out.
Thanks!
I am looking for advice from anyone who has experience of using the TruSeq Custom Amplicon Library Preparation kit please! Part of the protocol requires a 96well heat block that will cool samples from 95C to 40C over 80 minutes. We don't have the reccomended incubator, and Illumina Tech Support have said we can't use a thermal cycler. Our hot blocks take 240 minutes to cool to 40C - way to long for the samples to work. Has anybody used this kit with anything other that the recomended Scigene Hybex microsample incubator?? - don't want to have to spend out to test the kit out.
Thanks!
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